Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure, and ultrasonic scattering

We present reflection-mode bioimaging system providing complementary optical, photoacsoutic and acoustic measurements by acoustic detector after each laser pulse. While the photons absorbed within the sample provide optoacoustic (OA) signals, the photons absorbed by the external electrode of a detector provide the measurable diffuse reflectance (DR) from the sample and the probing ultrasonic (US) pulse. …

Simultaneous photoacoustic and optically mediated ultrasound microscopy: phantom study

An experimental setup for combined photoacoustic (PA) and optically mediated ultrasound (US) microscopy is presented. A spherically focused 35 MHz polyvinylidene fluoride (PVDF) ultrasonic detector with a numerical aperture of 0.28, a focal distance of 9 mm, and a bandwidth (−6  dB−6  dB level) of 24 MHz was used to obtain PA and US data with a 3 mm imaging depth. A fiber-optic system was …