Enhancing optoacoustic mesoscopy through calibration-based
iterative reconstruction

Optoacoustic mesoscopy combines rich optical absorption contrast with high spatial resolution at tissue depths beyond reach for microscopic techniques employing focused light excitation. The mesoscopic imaging performance is commonly hindered by the use of inaccurate delay-and-sum reconstruction approaches and idealized modeling assumptions. In principle, image reconstruction performance could be enhanced by simulating the optoacoustic signal generation, propagation, and detection path. However, for most realistic experimental scenarios, the underlying total impulse response (TIR) cannot be accurately modelled. Here we propose to capture the TIR by scanning of a sub-resolution sized absorber. Significant improvement of spatial resolution and depth uniformity is demonstrated over 3 mm range, outperforming delay-and-sum and model-based reconstruction implementations. Reconstruction performance is validated by imaging subcutaneous murine vasculature and human skin in vivo. The proposed experimental calibration and reconstruction paradigm facilitates quantitative inversions while averting complex physics-based simulations. It can readily be applied to other imaging modalities employing TIR-based reconstructions.

Schematic representation of the proposed experimental calibration procedure. a) A microsphere is moved to different positions in the sensitivity field of the transducer using motorized stages. At each stage position laser and data acquisition are triggered using customized electronics. b) To decouple the acoustic and optical propagation problems, illumination is provided from the side while scanning over the microsphere which is embedded in an agar block to avoid motion artifacts. c) The 4D TIR is then sampled over a three-dimensional region covering 1 mm in the lateral direction and 3 mm in the axial direction around the focal point.

Urs A.T. Hofmann, Weiye Li, Xose Luís Dean-Ben, Pavel Subochev, Hector Estrada, Daniel Razansky Enhancing optoacoustic mesoscopy through calibration-based iterative reconstruction

Photoacoustics 28 (2022) 100405

Follow the link below to find out more: https://doi.org/10.1016/j.pacs.2022.100405

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